procedure for serum processing from whole blood

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Procedure for Serum Processing from Whole Blood

Feb 05, 2016 · Procedure for Serum Processing from Whole Blood 66-Serum Processing Whole Blood SOP-LTC-SOP-66v1-2016-02-05 Page 5 of 9 7.1.4 Blood collection tubes are available in:7.1.4.1 plastic or glass, 7.1.4.2 with or without clot activator, 7.1.4.3 with or without a thrombin-based clot activator, 7.1.4.4 with or without silicone coating. Plasma and Serum Preparation Thermo Fisher Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the resulting supernatant, designated serum, is carefully removed using a Pasteur pipette. Plasma is produced when whole blood is collected in tubes that are treated with an anticoagulant. Standard Operating Procedures for Serum and Plasma Serum provides the liquid portion of the blood without cells and clotting factors and, therefore, should contain proteins and other molecules that represent the whole body system. The cells and clotting factors must be removed from the blood sample by allowing adequate time for a Collection of Serum from Blood (Procedure) :15. Wash your hand frequently and properly after blood collection. 16. Make sure to separate serum from red blood cells within 60 minutes (1 hr) of venipuncture. 17. During the collection of serum using pasteur pipette, you should take care the tip of the pipette do not touch the lysed cells settled at the bottom of the tube. 18. C4. Procedure for serum and plasma separtionSection 10.22 Procedure for separating Plasma and Serum from whole blood Aim:Effective Separation of blood products Purpose:To standardize separating procedures so that research samples will be uniform in quality The decision to collect anticoagulated (plasma, buffy coat, RBC) or coagulated (serum, Standard Operating Procedure (SOP) for6.2.2. Whole blood is collected in Na citrate CPT tubes. Record and report the time when it is collected. 6.2.2.1. Store tubes in an upright position at room temperature (15 to 30°C) until centrifugation. 6.2.2.2. Gently invert the tubes eight to ten times to remix cells immediately before centrifuging. 6.2.2.3. Specimen-handling, Serum, Plasma, Whole blood :Draw 12 mL of whole blood for each 5 mL of serum or plasma needed. Collect in an appropriate collection tube. Centrifuge for at least 15 minutes at 2200-2500 RPM. Pipette the serum or plasma into a clean plastic screw-cap vial and attach the label. Do not transfer red cells to the vial. 3.7.2 Collection, Processing, and Shipping of Blood 3.7.2 Collection, Processing, and Shipping of Blood Samples I. PURPOSE 1. The Central Blood Analysis Laboratory (CBAL) will be responsible for special blood collection and handling protocols as well as training and QC monitoring at the Clinical Centers. The laboratory will also be responsible for performing assays and reporting results. 2. Best Practice for PBMC ProcessingWhole Blood Dilution Suitable for the selected Bovine Serum (FBS) 10 % Dimethyl sulfoxide (DMSO) Cryopreservation Solution (CPS) 16 . Addition of CPS IVQAC SOP #007_Whole Blood Processing IVQAC CRYO #014_Cryopreservation of PBMCs Obtained from Blood Collection, Shipment, Processing, and Storage Sep 01, 2006 · As part of the AACR Annual Meeting Methods Workshop Sample Collection, Processing, and Storage for Large-Scale Studies:Biorepositories to Support Cancer Research, blood specimen collection, processing, storage, and dissemination issues were discussed. Whole blood and blood fractions comprise a major portion of biospecimen collections for PBMC and Monocyte Isolation from Whole BloodAliquot 600ul of serum from red top tube into pre-labeled serum tubes. 8.3.4 Store in -80°C Freezer. 8.4 Ficoll Gradient:8.4.1 Traditional method:8.4.1.1 Add 20mls of Ficoll to new 50mL conical replicating number of blood tubes. E.x. 2 blood tubes, 2 Ficoll tubes. 8.4.1.2 Add up to 30mls 1X PBS to whole blood tubes. PR31 Protocol for Preparation of Blood plasma and serumBlood serum is blood plasma without fibrinogen or the other clotting factors. Process samples as soon as possible. If storage is necessary prior to processing, store the blood at Procedure 1. Draw whole blood into vacutainer tube(s) containing no anticoagulant. Draw approximately 2 PROCEDURE Blood Collection - DATE ADOPTED This procedure covers the collection, processing, and storage of whole blood, plasma, buffy coat and serum. It does not cover how to draw blood from humans or any assays performed with the blood or blood products after processing. Authority and Responsibility for SOPs 1. Collection of Serum from Blood (Theory) :Immunology

  • Why Use Serum For Studies?Serum CollectionAgents Used For Serum PreservationSerum includes antibodies, antigens, electrolytes, hormones and any exogenous substances such as drugs and microorganisms and all proteins expect that used in blood clotting. Thus it has applications not only in the field of clinical diagnostics, but also in research. A very simple example could be their use in the different types of ELISA tests. They are therefore used to detect the presence of a particular antibody, antigen, hormone, exogenous substances, etc. It has beeBiospecimen Processing and Submission Procedures SWOGUse 10 mL red or red/black marble top (SST) vacutainer tubes with no anticoagulant to collect whole blood for serum processing. If your site does not have the size vacutainer tubes required by the treatment protocol, other sized tubes may be used to collect the required/requested amount of blood (e.g., if 1 x 10 mL of blood is requested, then 2 Serum Isolation from Human Peripheral BloodWhole blood should be drawn in sodiumheparin tubes with a venoject vacuum system. 7.2 Sample Processing All handlings of the sample should be done in a biohazard safety cabinet will wearing gloves Store the serum at 80°C (room KC01.081.1). 8. Procedure 1. In a centrifuge capable of safely spinning blood tubes, spin the blood at roughly 2500 Sheffield Clinical Research Facilityprocedure when processing and aliquoting blood specimens. This will ensure that there is continuity and consistency when processing and aliquoting blood samples. Definition . Aliquoting is the removal of serum plasma from the whole blood product. Procedure . The investigator or delegated person should follow protocol specific guidelines for SOP COLLECTION OF BLOOD - Uroweb1.2 Processing of blood specimens Processing of Serum tubes (samples B01, B02):Rack the tubes upright at room temperature for 30 minutes (minimum 30 minutes, maximum 60 minutes) to encourage clotting. Invert the blood tubes gently (10 to

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